Colocalization
Description
Colocalization features quantify the degree to which two different spectral channels spatially co-occur within a segmented object. these measurements assess the relationship and correlation between different protein markers.
Features extracted
Correlation-based metrics
Feature |
description |
|---|---|
MEAN.CORRELATION.COEFF |
mean pearson correlation coefficient |
MEDIAN.CORRELATION.COEFF |
median pearson correlation coefficient |
MIN.CORRELATION.COEFF |
minimum correlation coefficient |
MAX.CORRELATION.COEFF |
maximum correlation coefficient |
Manders coefficients (M1 and M2)
Feature |
description |
|---|---|
MEAN.MANDERS.COEFF.M1 |
average fraction of channel 1 overlapping with channel 2 |
MEDIAN.MANDERS.COEFF.M1 |
median M1 coefficient |
MIN/MAX.MANDERS.COEFF.M1 |
min/max M1 values |
MEAN.MANDERS.COEFF.M2 |
average fraction of channel 2 overlapping with channel 1 |
MEDIAN.MANDERS.COEFF.M2 |
median M2 coefficient |
MIN/MAX.MANDERS.COEFF.M2 |
min/max M2 values |
Overlap coefficient
Feature |
description |
|---|---|
MEAN.OVERLAP.COEFF |
average overlap coefficient |
MEDIAN.OVERLAP.COEFF |
median overlap coefficient |
MIN/MAX.OVERLAP.COEFF |
min/max overlap coefficients |
K1 and K2 coefficients
Feature |
description |
|---|---|
MEAN.K1 |
average intensity correlation quotient for channel 1 |
MEDIAN.K1 |
median K1 coefficient |
MIN/MAX.K1 |
min/max K1 values |
MEAN.K2 |
average intensity correlation quotient for channel 2 |
MEDIAN.K2 |
median K2 coefficient |
MIN/MAX.K2 |
min/max K2 values |
Use cases
Colocalization features are useful for:
Determining protein-protein interactions
Assessing subcellular localization patterns
Identifying co-distributed cellular components
Quantifying marker overlap in immunofluorescence